CLP^F produce Mac-1⁺ B1b cells and Mac-1⁺CD11c⁺ dendritic cells, but not myeloid cells. (A) Congenic sublethally irradiated mice received transplants of 10³ CLP^F cells. Three weeks after the transfer spleens were analyzed for donor-derived cells. FACS plots shown are previously gated on recipient (left) or donor-derived cells (right) and are B220⁻CD19⁻Gr-1⁻. Percentage of Mac-1⁺CD11c⁺ dendritic cells are shown. (B) Analysis of Mac-1⁺ B-1b cell differentiation from CLP^F in the spleen 4 weeks after transplantation. Cells are pregated on CD5⁻, and the percentage of Mac-1⁺IgD⁻ B-1b cells are shown. Numbers on plots are percentages of gated cells. (C) Methylcellulose myeloid differentiation assay. c-Kit⁺Lin⁻Sca-1⁺Flk2⁺ (MPP^F) cells, CLP^F, and Flk2⁻ fraction cells (F⁻ frac.) from bone marrow were double sorted and plated out at 100 cells (MPP^F) and 500 to 1000 cells (CLP^F and F⁻ frac.) in triplicates in methyl cellulose medium containing SCF, Flt3L, GM-CSF, Epo, Tpo, IL-3, IL-6, and IL-11. The average number of colonies yielded and their lineage content as colony forming units (CFU) from 2 combined experiments are shown. G, granulocyte; E, erythrocyte; M, macrophage; B, B cell; and Σ, sum.