CD8+ T-cell reactivity in Tie2-LacZ mice. (A) Heart and thymus sections of naive Tie2-LacZ mice were stained for β-gal and CD8. Images were acquired with a 25×/0.65 objective (magnification: ×162). (B,C) C57BL/6 (B6) and Tie2-LacZ (T2) mice were infected intravenously with 106 pfu MCMV-LacZ. (B) Tetramer analysis for the indicated β-gal– and MCMV-derived M45 epitopes was performed on day 6 after infection. Mean percentage of tetramer-positive cells within the CD8 compartment are indicated (± SEM; n = 3-4). (C) Lysis of peptide-pulsed EL-4 cells by MCMV-LacZ-induced CTLs. On day 6 after infection, splenocytes from the indicated mouse strains were restimulated in vitro for 5 days with β-gal497-504, β-gal96-103, or M45985-993 peptide and tested in a standard chromium release assay.
