14,15-EET activates Src in HDMVECs. (A) Quiescent HDMVECs were treated with and without 14,15-EET (0.1 μM) for the indicated times, and cell extracts were prepared and analyzed by Western blotting for pSrc using its phosphospecific antibodies. The blot was reprobed with anti-Src antibodies for normalization. (B) The bar graph represents the quantitative analysis of the time course effect of 14,15-EET on tyrosine phosphorylation of Src in triplicate. *P < .01 vs control. Error bars represent SD.