14,15-EET induces VEGF expression in a time-dependent manner in HDMVECs. Quiescent HDMVECs were treated with and without 14,15-EET (0.1 μM) for the indicated time periods and either RNA was isolated and analyzed for VEGF mRNA levels by RT-PCR (A), cell extracts were prepared and analyzed for VEGF levels by Western blotting (B), or the culture medium was collected and assayed for released VEGF by ELISA (C). The values in the bar graph in panel C are the means plus or minus SD of 3 independent experiments. *P < .01 vs control.