The lack of effect of 14,15-EET on VEGF expression in HASMCs. Quiescent HASMCs were treated with and without 14,15-EET (0.1 μM) for the indicated time periods and either RNA was isolated and analyzed for VEGF mRNA levels by RT-PCR (A), cell extracts were prepared and analyzed for VEGF protein levels by Western blotting (B), or the culture medium was collected and assayed for released VEGF by ELISA (C). Error bars represent SD.