Allele-specific analyses of histone modifications. The promoter proximal region (A) and third exon (B) were analyzed in ΔLCR (Hbb^D)/WT (Hbb^S) heterozygous mouse spleen cells. Enrichments of the WT (▩) and ΔLCR (□) adult β-globin gene relative to myoD1 in sample materials relative to those in input materials are plotted. Each bar represents the average of multiple experiments. Error bars represent SD. (C) Allele-specific ChIP in primitive erythroid (EryP) cells derived from ΔLCR (Hbb^D)/WT (Hbb^S) ES cells. Enrichment of the WT (▩) and ΔLCR (□) βh1-globin gene relative to amylase in sample materials normalized to input DNA is shown. The regions analyzed were the promoter proximal and third exon of the βh1-globin gene. Each bar represents the average of multiple experiments. Error bars represent SD.