H3 K79 dimethylation levels in the WT/ΔLCR adult globin gene during erythroid maturation. (A) Expression analysis of the WT (▩) and ΔLCR (□) adult β-globin genes in fractionated ΔLCR (HbbD)/WT (HbbS) primary cells. Bars represent the percentages of expression compared with the level of the WT allele in fraction 1. Fraction names are indicated at the bottom. Each bar represents the average of multiple RT-PCRs. Error bars represent SD. (B,C) Real-time RT-PCR analyses for erythroid (B) and non-erythroid (C) genes of GFLC fractions. Experimental values are standardized by HPRT expression levels for each fraction. Standardized values are normalized to fraction 1 (B) and fraction 6 (C). Error bars are SD. (D) Allele-specific ChIP analysis of GFLC-sorted fractions. Enrichment of H3 K79 dimethylation in the third exon of the WT (▩) and ΔLCR (gray circles) adult β-globin genes relative to myoD1 is plotted. Each point represents the average of multiple ChIPs. Error bars represent SD. The gel image is shown in panel E.
