H3 K79 dimethylation levels in the WT/ΔHS23 adult globin gene. (A) WT and mutant β-globin gene loci. The Hbb^D allele is shown. Arrows are DNaseI hypersensitive sites in the LCR. Black boxes represent β-like globin genes. Numbers to the right indicate the levels of expression in primary cells as the percentages of the WT level. (B) Allele-specific analyses of H3 K79 dimethylation enrichment in ΔLCR (Hbb^D)/WT (Hbb^S) and ΔHS23 (Hbb^D)/WT (Hbb^S) heterozygous mouse fetal liver cells. Enrichment of the WT type (▩) and ΔLCR or ΔHS23 (□) adult β-globin gene relative to myoD is shown. The promoter proximal and third exon of the adult β-globin gene were analyzed. Each bar represents the average of multiple experiments. Error bars represent SD. The gel image is shown in panels C and D.