Figure 1
![Figure 1. Transcriptional profile of endothelial and smooth muscle markers in hMSCs. (A) RT-PCR analysis of endothelial markers (VE-cadherin and PECAM1) in hMSCs at baseline and in hMSCs cocultured for 3 days with MS1 mouse endothelial cell line. (B) RT-PCR analysis of smooth muscle markers' mRNA in human mesenchymal stem cells, human aortic vascular smooth muscle cells (CRL-1999), and human dermal fibroblasts (CRL-2575). Water was used as negative control. (C-F) Protein expression of smooth muscle markers in hMSCs was confirmed by immunohistochemistry (α-smooth muscle actin [C], SM22α [D], desmin [E], and NG2 [F], yellow; DAPI, blue). Scale bar represents 50 μm.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/111/9/10.1182_blood-2007-10-118273/4/zh80080818120001.jpeg?Expires=1735841126&Signature=IWpGTnrwY7rV6E9X8wYy2OLYqqI~8deSANeCGVHEizpQmgFY6i-3ndUSLETvS-OrHRxOEa7oZicel9lCUEGq0iSFeFb82CPhwZNmLydfHUgvqAE6Ep3-XeL3yIFnA1J~wLjX7t~xeafeUfTV-3MC6vu-mabp49yQiG5fg0DvuPA9Je5kW1VywLCzPbqY32LFrjj7Ht9RjGXdV6mUmV8Jh3FxuHaH2mUfNQcCz8hqxqPcFBfpiojZNLx3vtrCG8scZESnDuMQVMKvW43E6s67W4crVhayG3Dk~upq2DiKL8DPwfy-qNZ0iFMqdqLowiq5IPbvdC67Li1u0BRECir9EA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Transcriptional profile of endothelial and smooth muscle markers in hMSCs. (A) RT-PCR analysis of endothelial markers (VE-cadherin and PECAM1) in hMSCs at baseline and in hMSCs cocultured for 3 days with MS1 mouse endothelial cell line. (B) RT-PCR analysis of smooth muscle markers' mRNA in human mesenchymal stem cells, human aortic vascular smooth muscle cells (CRL-1999), and human dermal fibroblasts (CRL-2575). Water was used as negative control. (C-F) Protein expression of smooth muscle markers in hMSCs was confirmed by immunohistochemistry (α-smooth muscle actin [C], SM22α [D], desmin [E], and NG2 [F], yellow; DAPI, blue). Scale bar represents 50 μm.
