American Society of Hematology

Figure 4

$Figure 4. The DDX3Y-encoded H-Y antigen recognized by CTL 68H7-819 is expressed on the surface of HLA-B*2705+ leukemic cells. CTL 68H7-819 recognition of primary leukemic samples from males carrying the HLA-B*2705 allele was tested by IFN-γ ELISA after overnight coculture with CTL at a CTL/leukemia ratio of 1:5. The 6 samples consisted of BMMCs derived from the hematopoietic cell transplant recipient from whom CTL 68H7-819 was derived (Recip ALL), PBMCs from one patient each with primary refractory AML and with CML in T-lymphoid blast crisis (CML-BC), and 3 PBMC samples from CLL patients. Negative controls included a CLL sample that did not carry the HLA-B*2705 allele, and CTL 68H7-819 alone.$

The DDX3Y-encoded H-Y antigen recognized by CTL 68H7-819 is expressed on the surface of HLA-B*2705⁺ leukemic cells. CTL 68H7-819 recognition of primary leukemic samples from males carrying the HLA-B*2705 allele was tested by IFN-γ ELISA after overnight coculture with CTL at a CTL/leukemia ratio of 1:5. The 6 samples consisted of BMMCs derived from the hematopoietic cell transplant recipient from whom CTL 68H7-819 was derived (Recip ALL), PBMCs from one patient each with primary refractory AML and with CML in T-lymphoid blast crisis (CML-BC), and 3 PBMC samples from CLL patients. Negative controls included a CLL sample that did not carry the HLA-B*2705 allele, and CTL 68H7-819 alone.

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