Generation of expanded T lymphocytes (T-APCs) as stimulator cells using anti-CD3 antibody (OKT3) and interleukin-2 (IL-2). (A) Overlay histograms of up-regulation of MHC class II and the costimulatory molecules CD80 and CD86 as determined by flow cytometry in gated CD3 cells for the stimulator used in Exp's VII-IX. (B) Fractions of positive cells for CD4, CD8, CD80, CD86, and HLA-DR in CD3⁺ T cells during the expansion process for the stimulator used in Exp's VII-IX. (C) Numeral expansion of T cells in all experiments performed.