Analysis of hDectin-1 protein levels in MDCs exposed to TLR agonists. Western blotting was performed to examine the hDectin-1b protein expression using a polyclonal rabbit anti–hDectin-1 antibody. (A) Lysates from immature and matured (with different compounds: pam₃cys, 5.0 μg/mL; poly I:C, 50.0 μg/mL; and R848, 2.0 μg/mL) MDCs are shown. (B) Titration with increasing amounts of poly I:C. The blots were reprobed with a GAPDH-specific antibody to confirm equal loading. The data shown are representative of 3 independent experiments.