Increase of 8972-Da peak in mouse GVHD plasma. (A) Representative SELDI spectra of the 8972-Da peak at day 0 obtained from pretransplantation and GVHD plasma (days 7, 14, 21, and 28 after transplantation) from an individual mouse are shown. The m/z range of 6000 to 10 000 m/z is shown. The box highlights an 8972-Da peak that is increased in intensity in GVHD plasma compared with day-0 samples. Number at the top and bottom of the figure indicates m/z. The left column indicates relative intensity of the ion peak. The Peak intensity of 8972 Da is 4.6, 47.3, 51.4, 25.2, and 55.9 on days 0, 7, 14, 21, and 28, respectively. (B) Mean normalized intensity values for 8972-Da peak in samples at each time point (day 0 is before transplantation; days 7, 14, 21, and 28 are after transplantation). The average intensity ± SE is depicted (n = 9). All GVHD samples (closed column except day-0 samples) were significantly higher than that of syngeneic controls (open column) on posttransplantation days 7, 14, 21, and 28 as judged by an unpaired t test. Increases on days 7, 14, 21, and 28 of allogeneic samples were also significantly higher than that of day-0 samples (before transplantation) (paired t test). Asterisk indicates P value.