Corroboration of SAGE results by RQ-PCR quantification of TOSO and BCL-2. (A) Relative real-time quantification of TOSO. The results are shown as fold changes, where we used a pool of 12 cDNA from nCD19⁺ cells to calculate 2^−ΔΔCt. The results are plotted in log scale, with bars representing the median values; the P value was calculated using Mann-Whitney test. (B) Graphic correlation between TOSO and BCL-2 expression. The results of real-time PCR for 42 CLL samples are demonstrated as ΔCt values, showing a positive correlation between TOSO and BCL-2 (P < .001, and Spearman r = 0.7427).