American Society of Hematology

Figure 2

$Figure 2. CD40 surface expression levels on lipopolysaccharide (LPS)–activated dendritic cells. (A) Monocytes fractionated from peripheral blood mononuclear cells were stained with CD14, CD11c, and CD80 (green) and after 6 days of IL-4 and GM-CSF stimulation, a dendritic cell phenotype was observed (blue). (B) Monocyte-derived dendritic cells treated with (blue) and without (green) LPS were stained with CD40 antibody. A replicate experiment for the TNFRSF5 −1CT individual is shown (red) overlapping the previous experiment. (C) Representative plots of differences between lipopolysaccharide (LPS) untreated and treated CD40 peak levels (TNFRSF5 −1CC/−1CT n = 7, −1TT n = 4). Means (bars) with standard errors (boxes) and one SD (whiskers) are plotted. Data from −1CC and −1CT carriers were pooled due to similarities in their CD40 shifts.$

CD40 surface expression levels on lipopolysaccharide (LPS)–activated dendritic cells. (A) Monocytes fractionated from peripheral blood mononuclear cells were stained with CD14, CD11c, and CD80 (green) and after 6 days of IL-4 and GM-CSF stimulation, a dendritic cell phenotype was observed (blue). (B) Monocyte-derived dendritic cells treated with (blue) and without (green) LPS were stained with CD40 antibody. A replicate experiment for the TNFRSF5 −1CT individual is shown (red) overlapping the previous experiment. (C) Representative plots of differences between lipopolysaccharide (LPS) untreated and treated CD40 peak levels (TNFRSF5 −1CC/−1CT n = 7, −1TT n = 4). Means (bars) with standard errors (boxes) and one SD (whiskers) are plotted. Data from −1CC and −1CT carriers were pooled due to similarities in their CD40 shifts.

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