![Figure 3. Increased workers of endothelial damage in sph/sph mice. Plasma levels of sVCAM-1 (A), sP-sel (C), and sE-sel (E) are increased in sph/sph mice compared with control mice. Levels of soluble murine vascular cell adhesion molecule-1 (sVCAM-1, A), soluble P-selectin (sP-sel, C), and soluble E-selectin (sE-sel, E) were determined by ELISA of plasma samples from control wild-type (+/+, □), control heterozygote (sph/+, ), and sph/sph (■) mice. Data are presented as mean (± SEM) for the following numbers: (A) n = 7 +/+ [all more than 8 weeks of age], n = 7 sph/+[all more than 8 weeks of age], n = 6 sph/sph [all more than 8 weeks of age]; (C,E) n = 13 +/+ [n = 6, 6-8 weeks of age; n = 7, more than 8 weeks of age], n = 12 sph/+[n = 6, 6-8 weeks of age; n = 6, more than 8 weeks of age], n = 17 sph/sph [n = 9, 6-8 weeks of age; n = 8, more than 8 weeks of age]. No significant difference between age groups was noted for sP-sel and sE-sel values. *Significant difference between sph/sph and control (+/+, sph/+) values: P < .0001 for sVCAM-1 (A) and sP-sel (C); P < .005 for sE-sel (E). Levels of sVCAM-1 (B), sP-sel (D), and sE-sel (F) in the plasma of sph/sph and control mice are variably reduced by ultracentrifugation. Plasma from +/+ (□ and gray-striped bars) mice more than 6 weeks of age and sph/sph mice (black and black-striped bars) more than 8 weeks of age was subjected to ultracentrifugation to remove microparticles as described under “Methods.” Levels of sVCAM-1 (B), sP-sel (D), and sE-sel (F) in plasma were compared before (“−UCS,” open and black bars) and after (“+UCS,” gray-striped and black-striped bars) ultracentrifugation. The difference between sVCAM-1 levels in A and B and sP-sel levels in C and D reflects the fact that plasma samples from separate sets of +/+ and sph/sph mice were used for the assays. Data are presented as mean (± SEM) for the following numbers: (B) n = 4 +/+ and n = 3 sph/sph; (D) n = 3 +/+ and n = 3 sph/sph; (F) n = 3 +/+ and n = 4 sph/sph. *Significant difference between “−UCS” and “+UCS” values, P < .03.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/112/2/10.1182_blood-2007-12-126714/4/zh80150821300003.jpeg?Expires=1735508001&Signature=X2qD86yzSl77rVBiByT0TKDr-Q7Sjfm2Rmya3U9fw~mgOhAJ7qK4ENplqfmT7g2kKHkLvW69oL-9y790AxMPpa5q8pmBd0NMA~qa9vr6psbT3n9avjnth-~ZkKM~JzLhCZFKDkh0p2Uj6tj5FN0Ti0QHmvXRnaIvJOVjxUNIGyufa0BHuMeIuBts-P2y4M~LhvEAYUT~QPz6QW9FIDxeRxL-HSJai25rAHUDj2mJA~wdA3binqQk5LIYyvQkEwNaJVHwnPw-jIa6G4R81s5O44K3yTzlF2CQvR3eFLYriGCprTi3Gp6LWdJCdrDa3xPQTRnyjjn2bli7aw~bnl3r7g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Increased workers of endothelial damage in sph/sph mice. Plasma levels of sVCAM-1 (A), sP-sel (C), and sE-sel (E) are increased in sph/sph mice compared with control mice. Levels of soluble murine vascular cell adhesion molecule-1 (sVCAM-1, A), soluble P-selectin (sP-sel, C), and soluble E-selectin (sE-sel, E) were determined by ELISA of plasma samples from control wild-type (+/+, □), control heterozygote (sph/+, 
), and sph/sph (■) mice. Data are presented as mean (± SEM) for the following numbers: (A) n = 7 +/+ [all more than 8 weeks of age], n = 7 sph/+[all more than 8 weeks of age], n = 6 sph/sph [all more than 8 weeks of age]; (C,E) n = 13 +/+ [n = 6, 6-8 weeks of age; n = 7, more than 8 weeks of age], n = 12 sph/+[n = 6, 6-8 weeks of age; n = 6, more than 8 weeks of age], n = 17 sph/sph [n = 9, 6-8 weeks of age; n = 8, more than 8 weeks of age]. No significant difference between age groups was noted for sP-sel and sE-sel values. *Significant difference between sph/sph and control (+/+, sph/+) values: P < .0001 for sVCAM-1 (A) and sP-sel (C); P < .005 for sE-sel (E). Levels of sVCAM-1 (B), sP-sel (D), and sE-sel (F) in the plasma of sph/sph and control mice are variably reduced by ultracentrifugation. Plasma from +/+ (□ and gray-striped bars) mice more than 6 weeks of age and sph/sph mice (black and black-striped bars) more than 8 weeks of age was subjected to ultracentrifugation to remove microparticles as described under “Methods.” Levels of sVCAM-1 (B), sP-sel (D), and sE-sel (F) in plasma were compared before (“−UCS,” open and black bars) and after (“+UCS,” gray-striped and black-striped bars) ultracentrifugation. The difference between sVCAM-1 levels in A and B and sP-sel levels in C and D reflects the fact that plasma samples from separate sets of +/+ and sph/sph mice were used for the assays. Data are presented as mean (± SEM) for the following numbers: (B) n = 4 +/+ and n = 3 sph/sph; (D) n = 3 +/+ and n = 3 sph/sph; (F) n = 3 +/+ and n = 4 sph/sph. *Significant difference between “−UCS” and “+UCS” values, P < .03.
