In vivo reconstitution of myeloid and lymphoid lineages using marrow cells from WT or FX^−/− mice injected into FX^−/− and WT recipients, and marrow cells from Fuc-T^−/− mice injected into Fuc-T^−/− recipients. (A) Schematic representation of the transplantation protocol. (B,C) Analysis of peripheral neutrophils (Gr-1⁺) and lymphocytes (B220⁺ and CD3ϵ⁺), and bone marrow myeloid progenitor cells by FACS 4 months after transplantation. Data are means plus or minus SD. Student t test was performed to compare the neutrophil or lymphocyte numbers in each transplant setting with those of WT to WT. P > .5 unless otherwise indicated. (D) One month after transplantation, WT recipient mice receiving donor cells from WT or FX^−/− mice were injected intraperitoneally with BrdU and fed with water containing BrdU for 3 days. The percentage of CMP and GMP cells (Lin⁻c-kit⁺Sca-1⁻IL7R⁻CD34⁺; numbers on graphs) in S-G₂/M phase of the cell cycle was analyzed by anti-BrdU and 7-amino-actinomycin D.