Figure 4
![Figure 4. Roles of the PI-3 kinase and the SAPK/JNK and ERK1/2 MAPK pathways in Ang-1–induced IL-8 mRNA and protein expression. (A,B) Effects of wortmannin (WM, 50 nM), LY294002 (10 μM), SP600125 (15 μM), and PD98059 (30 μM) on IL-8 mRNA levels (representative blots in panel A and means [± SEM] in panel B) measured after 1 hour of Ang-1 exposure. Vertical lines in panel A indicate that lanes were not directly adjacent to each other in the original blots. (C) Mean (± SEM; n = 3) of IL-8 protein measured after 12 hours of Ang-1 (expressed as 100%) or Ang-1 plus U0126 (30 μM), PD98059 (30 μM), LY294002 (10 μM), or SP600125 (15 μM). *P < .05 compared with Ang-1 alone.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/111/8/10.1182_blood-2007-08-110338/6/zh80080818040004.jpeg?Expires=1735844175&Signature=O2uxi5jermkR~ZDBP6bUdxzHBnfQE2To1nJY~n4WX5dgMW2rpVpfXxIjP4hxF6v8GJEZxnZbHiuSoJ-2JkzvhJUEM5oEvc27iBH8zxQNsC6qbJxt-Dcd7Aukrcpla0prPPD1SsDODonH2dI0MieQxP1NfAg-HWYYkR6UiTD260nLBHrdR16Mmn7nL20PWUP3dt1nlfRPn-Rl0bWo50XAUiRypVS0MWJmviUgCkBmQNiVHMU0Wzsl05enUQGFnPREOKpRQ26RXqOWW4BTRhi~qFHj7EJfMK14WqvbwS9jQkVQYGy8gzIBunuGH5n1yggqboct30EUDquF5xywKdD9fw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Roles of the PI-3 kinase and the SAPK/JNK and ERK1/2 MAPK pathways in Ang-1–induced IL-8 mRNA and protein expression. (A,B) Effects of wortmannin (WM, 50 nM), LY294002 (10 μM), SP600125 (15 μM), and PD98059 (30 μM) on IL-8 mRNA levels (representative blots in panel A and means [± SEM] in panel B) measured after 1 hour of Ang-1 exposure. Vertical lines in panel A indicate that lanes were not directly adjacent to each other in the original blots. (C) Mean (± SEM; n = 3) of IL-8 protein measured after 12 hours of Ang-1 (expressed as 100%) or Ang-1 plus U0126 (30 μM), PD98059 (30 μM), LY294002 (10 μM), or SP600125 (15 μM). *P < .05 compared with Ang-1 alone.
