Human lymphohematopoietic cell reconstitution in human Thy/Liv/CD34⁺ FLC-grafted NOD/SCID mice. (A) Levels of total human lymphohematopoietic (CD45⁺) cells, CD3⁺ T cells, and CD19⁺ B cells in PBMCs were analyzed by flow cytometry at week 9 after human tissue/cell transplantation. ■ and □ represent hu-mice that were subsequently used for immunization with DNP₂₃-KLH (n = 7) or adjuvant alone (ie, PBS controls; n = 5), respectively. (B,C) Levels of human CD3⁺ cells (B) and human CD19⁺ cells (C) in PBMCs, spleen, and LNs of DNP₂₃-KLH–immunized (n = 7) and PBS-injected control (n = 5) hu-mice were analyzed by flow cytometry at time of death (ie, week 2 or week 4 after booster immunization). Error bars represent SEM. (D) White pulp formation in hu-mouse spleen. Shown are sections prepared from a representative hu-mouse spleen stained with hematoxyin and eosin, anti-human CD3, CD20, and CD68.