Antigen-specific T-cell and antibody responses in immunized hu-mice. (A) Proliferation of human CD3⁺ T cells in response to KLH (left) and Con A (right). Stimulation index of each individual hu-mouse in DNP₂₃-KLH–immunized (●) and control (○) groups are shown. (B) Representative histograms showing CFSE levels in gated human CD3⁺ T cells from DNP₂₃-KLH–immunized (top) and control (bottom) groups. Cells stimulated with KLH, Con A, and medium are shown in left, middle, and right panels, respectively. (C) Serum levels of DNP-specific IgG in DNP₂₃-KLH immunized (●) and PBS control (○) mice at week 1 after booster immunization (left) and at time of death (ie, 2 or 4 weeks after booster immunization; right). Each symbol represents an individual hu-mouse. (D) Serum levels of DNP-specific human IgG1, IgG2, IgG3, and IgG4 in DNP₂₃-KLH–immunized (●) and PBS control (○) hu-mice at time of death (ie, 2 or 4 weeks after booster immunization). Each symbol represents an individual mouse. Horizontal lines in Figure 2A,D represent mean values.