Effect of citrullination on heparin binding properties of CXCL10 and CXCL11. (A,B) GAG binding was evaluated by immobilizing heparin on EpranEx plates, followed by adding a series of dilutions of CXCL10 (♦) or CXCL10-Cit5 (□) from 6 to 12 independent experiments depicted in panel A, or CXCL11 (♦) or CXCL11-Cit6 (□) from 3 to 6 independent experiments shown in panel B. GAG binding (mean ± SEM) was detected by chemokine-specific biotinylated antibodies and shown as the percentage binding of 10 nM of CXCL10 in panel A (SEM control was 1.07%) and of 100 nM of CXCL11 in panel B (SEM control was 1.24%). Statistical analysis was performed using the Mann-Whitney test (‡P < .05, ‡‡P < .01, ‡‡‡P < .001 for comparison of authentic with citrullinated chemokine).
