Expression of the transduced AML1-D171N, AML1-S291fs, and AML1 WT in spleen of the transplanted mice. (A) Lysates of spleen cells were immunoblotted with anti-Flag Ab. As a positive control, Plat-E packaging cells were transduced with mock (lane 1), AML1 WT (lane 2), AML1-D171N (lane 3), or AML1-S291fs (lane 4). Spleen cells were derived from mice/D171N (lanes 5-8), mice/S291fs (lanes 9-11), mice/WT (lane 12), mice/mock (lane 13), or control normal mouse (lane 14). White arrows indicate transduced AML1 WT, AML1-D171N, and AML1-S291fs. (B) AML1 WT-transduced cells were undetectable in PB at 1 month after the transplantation. Flow cytometric analysis of PB obtained from mice that received transplants of AML1-D171N, AML1-S291fs, AML1 WT, and mock at 1 month after transplantation.