polyP slows the rate of fibrinolysis. (A) Lysis of clots formed by preincubating 1.0 mg/mL fibrinogen for 15 minutes in the presence of 2.5 mM CaCl2 with 1 mM polyP (▼) or without polyP (○), after which 8 nM plasmin was added followed immediately by 1 nM thrombin. Data are mean plus or minus SE (n = 8). (B) Lysis of clots formed by preincubating 1.0 mg/mL fibrinogen and 200 nM plasminogen for 15 minutes in the presence of 2.5 mM CaCl2 with 1 mM polyP (▽) or without polyP (○), after which 375 pM tPA and 1 nM thrombin were added to initiate clotting. In both panels, absorbance data were normalized to the maximal A405 value for each curve, plotted versus time after reaching maximal A405. The insets show the entire turbidity profiles from the point of thrombin addition, without normalization. Data are mean plus or minus SE (n = 4).
