The combination of ABT-737 and bortezomib or carfilzomib significantly changes the mitochondrial membrane potential (ΔΨm) in DLBCL (RL) and MCL (HBL-2). (A) HBL-2 and RL cells were incubated with ABT-737 from 1 nM to 10 μM for 24 hours. (B) HBL-2 cells were incubated with with ABT-737 (10 nM), bortezomib (B, 6 nM), carfilzomib (C, 10 nM) for 24 hours. Both combination groups were statistically significant compared to any of the single groups and controls (P < .001). (C) RL cells were incubated with ABT-737 (100 nM), bortezomib (B, 10 nM) for 48 hours. In the sequence explored (>) the second drug was added after 24 hours from the beginning. All combination groups were statistically significant compared each single group and the control (P < .001). No statistically significant difference among the 3 combination groups explored. Δψm was evaluated by cytofluorimetric analysis of JC-1. Results represent the means plus or minus SD. cccp indicater carbonyl cyanide m-chlorophenylhydrazone.