Tx-Cre–mediated Gata1 deletion leads to anemia and thrombocytopenia in adult mice. (A) PCR analysis of recombination in DNA isolated from BM of Gata1^fl/y::Tx-Cre (KO-Tx, n = 4), Gata1^fl/y (WT, n = 3), and Tx-Cre (Cre, n = 1) mice. (B) Western blotting using anti-Gata1 antibody N6 (top) and Npm1 (bottom). Whole cell extracts were prepared from the BM of KO-Tx (n = 4), WT (n = 3), and Cre (n = 1) mice at week 4 of the Tx treatment. (C) Peripheral blood counts of KO-Tx mice (■) and WT mice (□) at 0, 3, and 4 weeks after the Tx treatment. Samples are collected from 10 to 15 mice per group. Results shown as mean plus or minus SD (error bars). WBC indicates white blood cell counts; RBC, red blood cell counts; HCT, hematocrit; PLT, platelet counts. (D) Erythropoietin (Epo) levels in plasma measured at weeks 3 and 4 after the start of Tx treatment in KO-Tx (●) and WT (○) mice. Epo values are plotted against HCT value for each mouse. (E) Bar graph representing the Log₁₀(Epo)/HCT ratio at weeks 3 and 4 of Tx treatment. Average and SD (error bars) of more than or equal to 4 mice per group are shown.