C-type lectin DCIR is expressed by human pDCs. PDCs were isolated with anti–BDCA-4 antibody-coated magnetic beads and labeled with the indicated antibodies (shaded areas) or the matching isotype controls (dotted lines). Samples were analyzed by flow cytometry and gated on the pDC population (according to forward/sideward scatter). DCIR and BDCA-2 are clearly expressed, whereas expression of the CLRs DC-SIGN, MR, and dectin-1 cannot be detected. FACS data are representative of 4 independent experiments.