Decreased expression of ISGs in APECED cell populations. Expression of ISGs (A) and other genes (E) in freshly isolated PBMCs from IFN-α antibody-positive (Ab⁺, 4 cases) and -negative (Ab⁻, 1 case) APECED patients and healthy controls (Ctrl, 5 cases). (C) Expression of ISGs in freshly isolated plasmacytoid DCs (2 cases in each group). (B) Expression of ISGs in freshly isolated monocytes from IFN-α antibody-positive (6 cases) and -negative (1 case) APECED patients and healthy controls (7 cases). (D) Expression of ISGs in monocyte-derived DCs from APECED patients and healthy controls (3 cases in each group). *P < .05, **P < .001, ***P < .001, comparison of the Ab⁺ APECED patient and control blood cells. Bars represent group averages.