aCGH analyses of SRS19-6–induced leukemias. (A) Splenic tumor DNA from mouse 540 (AML; Cebpa^BRM2/BRM2) was subjected to aCGH analysis as described in “Detection of chromosomal copy number aberrations by array CGH.” The resulting profiles are displayed using a moving average of 3 (black dots) with the smoothed values superimposed (red lines). The resulting profile demonstrates an essential normal karyotype. (B) aCGH analysis of splenic tumor DNA from mouse 341 (AML; Cebpa^+/BRM2) demonstrates gain of chromosomes 6, 12, and 15. The arrow indicates a small region at the tip of chromosome 11 that is deleted in 5 of 10 tumors of various genotypes and phenotypes (C). The insert highlights this small region. Here, raw normalized values are displayed without moving average, with smoothing. (C) ENSEMBL screenshot showing the genes located at the tip of chromosome 11 (due to the repetitive nature of the acromeric region, there are no probeset on the arrays upstream from 3002 kb). The lines above indicate the extent of the deletion and the identity of the mice that have a deletion in this area. The minimal deletions pinpoint Sfi1 and Eif4enif1 as candidate tumor-suppressor genes.