Nonadherent bone marrow cells can produce stable hematopoietic engraftment. (A) GFP expression by white blood cells (WBC), red blood cells (RBC), and platelets (PLT) assessed by flow cytometry with the whole peripheral blood analyses. The findings are reported as mean (+SD) percentages of GFP⁺ cells representing each of the 3 hematopoietic lineages. Values for all lineages were significantly increased at 24 weeks (WBC, P < .001; RBC, P = .03; PLT, P = .008) compared with 2 weeks, becoming stable thereafter. (B) Flow cytometric analysis of GFP expression in whole peripheral blood (PB) collected from the mice that underwent transplantation (n ≥ 4 per group) at increasing times after transplantation. The findings are reported as mean (+SD) percentages of GFP⁺ cells. (C) GFP expression in bone marrow assessed at different times after transplantation. We scored 20 randomly selected 400× fields in representative sections from each mouse (n ≥ 4 mice per group). The values were derived from immunohistochemical analyses of bone/bone marrow sections stained with anti-GFP antibody and are expressed as mean (+SD) percentages of GFP⁺ cells in each microscopic field. All experiments were performed in triplicate.