Phenotypic features of MoDCs, mDCs and tolDCs, generated from human monocytes. (A) Surface expression of differentiation markers (solid lines) was assessed by FACS analysis. Shaded regions represent staining with isotype control antibodies. The numbers represent MFI values of the respective markers. The data are representative of the results of 5 separate experiments. (B) Cytokine synthesis by the DCs was evaluated using culture supernatants of MoDCs or tolDCs stimulated with or without LPS for 24 hours. Results are the mean concentration plus or minus SD of triplicate experiments. (C) Cytokine synthesis by the DCs was evaluated using culture supernatants of MoDCs or tolDCs stimulated with E coli DNA or calf thymus DNA for 24 hours. Results are the mean concentration plus or minus SD of triplicate experiments.