Activation of iNKT cells by α-GalCer presented by distinct DC subsets. (A) Cytokine production of primary iNKT cells was assessed in culture supernatants of PBMCs after 72 hours of stimulation with distinct subsets of syngeneic DCs pulsed with α-GalCer (αGC) or vehicle (Veh). Results are the mean concentration plus or minus SD of triplicate experiments. (B) Mean plus or minus SE fold expansion of total iNKT cells after 7 days of stimulation of PBMCs with distinct subsets of syngeneic DCs loaded with α-GalCer (αGC) or vehicle (Veh) in the presence of anti–IL-10 mAb or rat control IgG1 from 3 different subjects (*P < .05). (C) Detection of apoptosis of PBMCs cocultured with α-GalCer-pulsed DCs (MoDCs, mDCs, or tolDCs) for 24, 48, or 72 hours. The percentage of cell death was measured by annexin V and PI staining using FACS analysis.
