Suppressor function of Vα24⁺ iNKT cells against the proliferation of alloreactive CD4⁺ T cells. 5 × 10⁴ allogeneic CD4⁺ T responder cells from 3 different subjects were labeled with CFSE and cultured with 10⁴ α-GalCer– or vehicle-loaded MoDCs and 5 × 10⁴ moDC-NKT or tolDC-NKT cells in the presence of anti-IL-10 mAb or rat control IgG₁. Cultures without Vα24⁺ iNKT cells were used as a control. Five days later, cell division in the cultures was analyzed using flow cytometry. Dot plots show CFSE fluorescence (x-axis) vs CD4 expression (y-axis) gated on CFSE-labeled CD3⁺CD4⁺ T cells. The percentage of live divided cells is shown in the table below the flow cytometry plots.