Effect of Vα24+ iNKT cells on DC maturation. MoDC-NKT or tolDC-NKT cells were cultured with α-GalCer– or vehicle-loaded MoDCs for 48 hours. (A) Allogeneic MLR. MoDC-NKT or tolDC-NKT cells were cultured with α-GalCer–loaded MoDCs for 48 hours. After removing Vα24-positive cells, DCs isolated from cultures of MoDCs with moDC-NKT or tolDC-NKT cells were cultured with allogeneic PBMCs for 5 days in MLR assays. The results shown represent one of 2 experiments. (B) DC phenotype in subjects 1 and 2. MoDC-NKT or tolDC-NKT cells were cultured with α-GalCer (αGC)- or vehicle (Veh)-loaded MoDCs for 48 hours (subject 1) in the presence of anti–IL-10-blocking mAb or rat control IgG1 (subject 2). Cell surface markers displayed by the DCs were assessed by FACS. The numbers represent the geometric MFI of the respective markers.
