Array CGH and FISH correlation of 1p36.3 and 6q23.3. (A) FISH validation of the 1p36.22-p36.33 region (ID no. 1), which presented significant correlation with clinical outcome. Array CGH (top panel) and FISH (bottom panel) demonstrating a case without deletion at 1p36.3 (normal), a case with heterozygous deletion at 1p36.3, and a case with homozygous deletion at 1p36.3. The 1p36.32 probe was labeled red, while the control probe at 1q32 was labeled green. (B) A composite-array CGH ideogram profile of 6q alterations affecting at least 10% and at least 15% of FL cases was generated by intersection analysis. The alteration indicated by the black box in the 15% ideogram corresponds to the 6q23.3 region targeted for FISH validation. (C) FISH validation of the 6q23.3 region (ID no. 20), which presented significant correlation with clinical outcome. Array CGH (top panel) and FISH (bottom panel) demonstrating a case with homozygous deletion and a case with homozygous deletion at 6q23.3 and proximal and distal heterozygous deletion. The 1p36.3 and 6q23.3 probes were labeled red, while the control probes at 1q32.3 and 6p12.3 were labeled green. Green arrows in FISH indicate the presence of heterozygous deletion, while yellow arrows indicate the presence of homozygous deletion. For array CGH, each dot represents a BAC clone and the light blue lines represent visual calling of aberrations. Loss is indicated by a shift to the left of center and gain by a shift to the right of center. Vertical lines are −1 and + 1 scale bars of log₂ ratios. “Comp” refers to the HMM computational analysis of aberration frequency.