Figure 1
Figure 1. Effect of tPA deficiency on Akt phosphorylation in the ischemic brain. (A) Representative Western blot analysis for Akt phosphorylated at serine 473 (pAkt) and total Akt in brain extracts from wild-type (WT) and tPA-deficient (tPA−/−) mice 1 to 24 hours after middle cerebral artery occlusion (MCAO). S denotes sham-operated mice. (B) Mean density of the band of 6 immunoblots for pAkt in wild-type (□) and tPA−/− mice (■) 1 to 24 hours after MCAO. n = 6. Lines depict SD. *P < .001 relative to either WT or tPA−/− sham-operated mice. †P < .001 compared with tPA−/− mice 1 hour after MCAO. ‡P < .001 relative to WT mice 24 hours after MCAO. (C) Representative Western blot analysis for pAkt in brain extracts from tPA−/− mice 1 hour after middle cerebral artery occlusion (MCAO) and either no treatment (NT) or the intracerebral injection of tPA (tPA).

Effect of tPA deficiency on Akt phosphorylation in the ischemic brain. (A) Representative Western blot analysis for Akt phosphorylated at serine 473 (pAkt) and total Akt in brain extracts from wild-type (WT) and tPA-deficient (tPA−/−) mice 1 to 24 hours after middle cerebral artery occlusion (MCAO). S denotes sham-operated mice. (B) Mean density of the band of 6 immunoblots for pAkt in wild-type (□) and tPA−/− mice (■) 1 to 24 hours after MCAO. n = 6. Lines depict SD. *P < .001 relative to either WT or tPA−/− sham-operated mice. †P < .001 compared with tPA−/− mice 1 hour after MCAO. ‡P < .001 relative to WT mice 24 hours after MCAO. (C) Representative Western blot analysis for pAkt in brain extracts from tPA−/− mice 1 hour after middle cerebral artery occlusion (MCAO) and either no treatment (NT) or the intracerebral injection of tPA (tPA).

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