The effect of the interaction between tPA and LRP on pAkt is cell type– specific. (A,B) Representative Western blot analysis of pAkt and total Akt in extracts from astrocytic (A) and neuronal (B) cultures after 1 hour of exposure to oxygen-glucose deprivation (OGD) conditions and incubation with either tPA or a combination of tPA and the receptor-associated protein (RAP). RAP is a molecular chaperone that inhibits the binding of LRP to its ligands. NT indicates no treatment. Each observation was repeated 4 times.