ADAMTS13 antigen and proteolytic activity in murine plasma. (A) Calibration curve using diluted wild-type murine plasma (0, 1:80, 1:40, 1:20, and 1:10) that corresponds to 0, 0.0125, 0.025, 0.05, and 0.10 μg/mL ADAMTS13 antigen as shown in the x-axis. (B) Plasma levels of ADAMTS13 antigen in various mice at 1, 3, and 5 months after transplantation compared with those in mice without transplantation (Pre). N1 through Q2 represent difference mice. (C) Cleavage of GST-vWF73 by wild-type murine plasma (0, 0.25, 1.25, and 2.5 μL) and by plasmas (5 μL, each) from recipient mice at 1 month of posttransplantation with HPCs transduced with the vector encoding GFP alone (HPC + GFP, lanes O1-O4) as negative controls (top). A representative calibration curve is shown (the fluorescence intensity against μL of murine plasma added; bottom). (D) Cleavage of GST-vWF73 by murine plasma (5 μL) at 1, 3, and 5 months after transplantation of HPCs transduced with the vector encoding GFP-mAdamts13 (N1-N3, P1-P4, and Q1-Q2). Arrowhead indicates the cleavage product of 34.4 kDa.