Figure 3
Figure 3. Role and signaling of IGF2 in EPC chemotaxis and adhesion. (A,B) EPCs were treated with IGF2 (1, 10, 50, and 10 ng/mL). (C,D) EPCs were preincubated for 30 minutes with or without 5 mM M6P or IGF2RN (2.5, 5 μg/mL) and stimulated with 50 ng/mL IGF2. After 30 minutes, adhesion was quantified by counting the cells that attached to the fibronectin-coated matrix with optical microscopy at 200× magnification. (E,F) EPCs were preincubated for 6 hours with or without 50 ng/mL PTX or preincubated for 30 minutes with or without 5 μM U73122, 5 μM U73343, or 5 μM BAPTA-AM and stimulated with 50 ng/mL IGF2. After 24 hours, chemotaxis was quantified by counting the cells that migrated to the lower side of the filter with optical microscopy at 200× magnification (E). After 30 minutes, adhesion was quantified by counting the cells (F). Data are the mean plus or minus SE. **P < .01 versus IGF2 alone.

Role and signaling of IGF2 in EPC chemotaxis and adhesion. (A,B) EPCs were treated with IGF2 (1, 10, 50, and 10 ng/mL). (C,D) EPCs were preincubated for 30 minutes with or without 5 mM M6P or IGF2RN (2.5, 5 μg/mL) and stimulated with 50 ng/mL IGF2. After 30 minutes, adhesion was quantified by counting the cells that attached to the fibronectin-coated matrix with optical microscopy at 200× magnification. (E,F) EPCs were preincubated for 6 hours with or without 50 ng/mL PTX or preincubated for 30 minutes with or without 5 μM U73122, 5 μM U73343, or 5 μM BAPTA-AM and stimulated with 50 ng/mL IGF2. After 24 hours, chemotaxis was quantified by counting the cells that migrated to the lower side of the filter with optical microscopy at 200× magnification (E). After 30 minutes, adhesion was quantified by counting the cells (F). Data are the mean plus or minus SE. **P < .01 versus IGF2 alone.

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