DCs are superior to monocytes for the expansion of TH17 cells. T cells were labeled with the CFSE dye to monitor proliferation and cultured either alone (T alone) or with cytokine-matured DCs (T + Cyt DC) or monocytes (T + monocyte). On day 5, some of the cells were examined for proliferation as well as IL17 production using PMA/ionomycin stimulation as before. IL2 was added to the rest of the cultures. On day 12, the DC–T-cell cocultures were again examined for proliferation of the T cells as measured by the CFSE dilution and the presence of IL17 cells as well as for the cytokine profile of the IL17-producing cells with PMA/ionomycin stimulation. Figure represents one of 5 similar experiments.