Mechanism of activation of Th17 cells by DCs. T cells were stimulated with cytokine-matured DCs alone or in the presence of blocking antibodies against IL1b, IL6, and IL23p19 or isotype antibodies. In some conditions, the T cells were separated from DCs using transwells. At 5 days after coculture, the T cells were stimulated with PMA/ionomycin to assess the presence of IL17-producing cells. The figure shows the percentage of decrease in IL17 cells when the DCs are separated from the T cells by transwell as well as in the presence of the blocking antibodies (n = 2). Figure shows mean plus standard deviation.