Expansion of antigen-specific Th17 cells by DCs. (A) Comparison of monocytes versus Cyt-DCs for the expansion of antigen-specific Th17 cells. CFSE-labeled T cells were cultured alone (T cells), or cocultured with cytokine-matured DCs (T cells + Cyt DC) or monocytes (T cells + Mono) with or without loading with Candida antigen (20 μg/mL overnight). At 5 days later, T cells were stimulated with PMA/ionomycin in the presence of monensin. Candida antigen–specific T cells (those that proliferated and diluted their CFSE in response to stimulation with DCs loaded with Candida antigen) were examined for the production of IL17 and IFNγ as well as IL2 using flow cytometry. Figure represents one of 5 similar experiments. (B) T cells from cultures expanded using Candida-loaded Cyt-DCs as in panel A were restimulated with unpulsed DCs or Candida-loaded DCs. The presence of IL17-, IFNγ-, and IL2-producing cells was analyzed using flow cytometry.