Figure 5
Activity of human rFVa C1 and C2 domain mutants in the presence of C6PS. The cofactor activity of rFVa mutants was determined as described in the equation 1 in “Lipid-dependent cofactor activity.” Reaction mixtures contained 1 μM prothrombin, 1 nM FXa, 5 nM rFVa, 5 μM DAPA in 50 mM Tris, 150 mM NaCl, 5 mM Ca2+, pH 7.5. Shown are means (± SD) of 4 measurements. *P < .002 compared with wild-type rFVa.
