Notch1 expression is required for LMP2A to alter mRNA levels of B cell–specific transcription factors in TgE mice. Real-time RT-PCR gene expression analysis was performed using total RNA isolated from B220⁺ bone marrow B cells harvested from methylcellulose cultures of each genotype. Primers specific for E2A and EBF were used along with GAPDH as a control for RNA levels. Fold change in gene expression was calculated using the ΔΔCt method, and fold change is shown relative to wild-type (Notch1^lox/lox) mice. Results are the average of at least 3 independent experiments plus or minus SE. Notch1^lox/lox, n = 6 mice; Notch1^lox/lox CD19^+/Cre, n = 4; TgE Notch1^lox/lox, n = 3; TgE Notch1^lox/lox CD19^+/Cre, n = 6.