TRAF2 expression decreases along differentiation into macrophages. (A) Immunoblot analysis of TRAF2 expression in peripheral blood monocytes exposed to 100 ng/mL M-CSF and in THP-1 and U937 cells exposed to 20 nM TPA for the indicated times (sample A and sample B represent 2 independent experiments). HSC70 indicates loading control. (B) Immunoblot analysis of TRAF2 expression in THP-1 cells exposed for the indicated times to 20 nM TPA. In indicated samples (+MG), 30 μM MG132 was added 7 hours before preparing extracts and immunoblot analysis, ie, 16 hours after initiation of the differentiation by TPA. HSC70 indicates loading control. (C) Real-time PCR analysis of mRNA expression of TRAF2 in peripheral blood monocytes exposed to 100 ng/mL M-CSF (mean ± SD of independent triplicates). AU indicates arbitrary units.