MSC-DCs up-regulate Jagged-2 expression. (A) FACS analysis for expression of notch ligands on MSC-DCs. Expression of Jagged-1, Jagged-2, and Delta-1 on the surface of imDC, maDC, MSC-DC, and activated MSC-DC (48 hours after MLC) were analyzed by flow cytometry. Dotted lines indicate background staining. Numbers in histograms indicate the geometric mean fluorescence of each DC population. (B) Western blot detection for notch ligands of MSC-DCs. The expression of Jagged-1, Jagged-2, and Delta-1 protein were further detected by Western blot, using 30 μg protein per lane of lysates from imDC, maDC, MSC-DC, and activated MSC-DC. Results are representative of at least 5 independent experiments.