Leukemia-associated antigen-specific CD8⁺ T cells preferentially localize to the bone marrow in patients with myeloid malignancies. Cognate D227K/T228A, WT and Q115E pHLA A*0201 tetramers were used to quantify CD8⁺ T-cell populations specific for CMV pp65_495-503, WT1_126-134 and PR1 in the peripheral blood (PB) and bone marrow (BM). Graphs depict the frequency of antigen-specific CD8⁺ T cells within each compartment that bind to each pHLA A*0201 tetrameric form. Post-HSCT samples are shown as triangles and pre-HSCT samples are shown as circles; horizontal bars represent median values. Data are color-coded to match the key shown in Table 1. All pre-HSCT samples were drawn from time points before the conditioning regimen; post-HSCT samples were drawn at a median of 7.5 months (range, 2 to 28 months) from the date of transplant. Multiple time points were assessed in some patients, indicated by identical symbols. In some cases, not all cognate tetrameric forms were used due to limited cell availability. The differences between PB and BM were significant for PR1-specific CD8⁺ T-cell populations quantified with all 3 cognate tetramers (P = .003 with WT and Q115E, P < .001 with D227K/T228A; Mann-Whitney); there were no significant differences between compartments with respect to CMV-specific CD8⁺ T-cell populations.