Establishment of an HLA-A*0201–restricted and Aur-A207-215 peptide–specific CTL line, AUR-1. The cytotoxicity of the CTL line designated AUR-1 against various LCLs and HLA-A*0201 gene–transfected cells (C1R-A*0201), which were loaded or unloaded with Aur-A207-215 peptide, was determined by 4-hour 51Cr-release assays at effector-to-target (E:T) ratios of 10:1, 5:1, and 2.5:1.