Double fluorescence staining of CD34⁺ endothelia (green label) and ED-B FN (red label) indicates colocalization of both antigens. The colocalization of CD34⁺ endothelia and ED-B FN was investigated by double fluorescence confocal microscopy of paraffin-embedded specimens of diffuse large B-cell lymphoma, follicular lymphoma (Grad 3a), and classic Hodgkin lymphoma. Colocalization of ED-B FN and CD34⁺ endothelia was found as indicated by the yellow label. Nuclei were stained blue (TOTO3). The asterisks in the right panels (classic Hodgkin lymphoma) indicate a large vessel lumen.