Therapeutic effects of vaccination. (A) Example of a clinical CR. (i) CT scan images of the chest of patient no. 12 revealed multiple small enlarged mediastinal and left axillary LNs (left, arrows) that normalized 6 months after vaccination (right). (ii) Involvement of BM (lanes 1 and 2: before vaccination and 6 months after vaccination, respectively) and PB (lanes 3, 4, 5, 6, and 7: before vaccination, and 3, 6, 12, and 18 months after vaccination, respectively) was evaluated by PCR amplification of the clonal tumor-specific IgH gene rearrangement using IgH-specific molecular probe. Actin amplification was performed as a control. Line 8 represents no DNA; M, marker. (B) Example of a clinical PR. (i) CT scan images of the retroperitoneum of patient no. 5 showed enlarged retroperitoneal LNs (left, arrows) that shrunk 4 months after vaccination (right). (ii) Involvement of BM (lanes 1, 2, and 3: before vaccination, and 4 and 12 months after vaccination, respectively) was evaluated by PCR amplification for panel A. Actin amplification was performed as a control. Line 4 represents no DNA; M, marker.