MMP-7 degrades human native sVEGFR-1 from endothelial cells. The supernatant of HUVECs was incubated with MMP-7 at the doses indicated. The digestion products were analyzed by SDS-PAGE and Western blotting using antibody against the extracellular region of VEGFR-1. ▶ indicates the putative band of full-length sVEGFR-1 that corresponds with the molecular mass of human native sVEGFR-1 from endothelial cells (110 kDa).¹⁶  → indicates the putative proteolytic fragments of sVEGFR1 from endothelial cells.